Eduardo José Xavier Rodrigues de Pinho e Melo
Professor associado com agregação
Faculdade de Ciências e Tecnologia
Centro de Ciências do Mar
Subsistema
Docentes Universitário
Unidade ID
Centro de Ciências do Mar
Regime
Exclusividade
Vínculo
CT em Funções Públicas por tempo indeterminado
Eduardo Pinho Melo. Concluiu o(a) Título de Agregado em Agregação em Biotecnologia em 2007 pelo(a) Universidade de Lisboa Instituto Superior Técnico, Doutoramento em Biotecnologia em 1995 pelo(a) Universidade de Lisboa Instituto Superior Técnico, Doutoramento em Biotecnologia em 1995 pelo(a) Universidade de Lisboa Instituto Superior Técnico e Licenciatura em Biologia Vegetal Aplicada em 1989 pelo(a) Universidade de Lisboa Faculdade de Ciências. Publicou 52 artigos em revistas especializadas. Atua na(s) área(s) de Ciências Naturais com ênfase em Ciências Biológicas.
Atividades
Atividades
2021 - 2021. Orientação. In vitro protein thiol oxidation by endoplasmic reticulum enzymatic pathways.Biological Engineering (Mestrado). Orientador.
2021. Apresentação oral de trabalho. Expression of the prion protein and membrane-enriched proteome changes
during neural differentiation and in neuroblastoma cells. 7th International Caparica Conference on Analytical Proteomics. Universidade Nova de Lisboa (Costa da Caparica).
during neural differentiation and in neuroblastoma cells. 7th International Caparica Conference on Analytical Proteomics. Universidade Nova de Lisboa (Costa da Caparica).
2020 - 2020. Arbitragem científica em conferência. 9th Iberian Congress on Prions. Corunha, Espanha.
2019. Apresentação oral de trabalho. Stability of protein formulations at sub-zero temperatures by isochoric cooling. Biological and pharmaceutical complex fluids III: Protein self-assembly, rheology and interfacial properties.
2018. Apresentação oral de trabalho. Live-cell imaging of protein disaggregation in the endoplasmic reticulum. 7th Iberian congress on prions.
2018 - 2018. Arbitragem científica em conferência. 7th Iberian Congress on Prions. Laguardia, Espanha.
2018 - 2018. Organização de evento. Organizing Committee Member of Prion 2018 (2018). Universidade de Santiago de Compostela.
2016. Apresentação oral de trabalho. The Yin and Yang of Protein Aggregation. ProtStab 2016, 11th International Conference on Protein Stabilisation.
2016 - 2016. Arbitragem científica em conferência. 5th Iberian Congress on Prions. Barcelona, Espanha.
2012 - 2016. Orientação. Development of Technology for cryopreservation and formulation of therapeutic proteins.Chemical Engineering (Doutoramento). Coorientador.
2015. Apresentação oral de trabalho. Getting proteins more compact prevents protein amyloidosis. 4th Iberian Congress on Prions.
2015. Apresentação oral de trabalho. Membrane-enriched proteomic changes associated with prion protein expression during neural differentiation and in neuroblastoma cells. 4th Iberian Congress on Prions.
2015 - 2015. Arbitragem científica em conferência. 4th Iberian Congress on Prions. Lisboa, Portugal.
2014. Apresentação oral de trabalho. Membrane proteome and prion protein profiling during neural differentiation of stem cells. 3rd Iberian Congress on Prions.
2014. Apresentação oral de trabalho. Osmolyte induce compaction of the protein tertiary structure preventing amyloid fiber formation and protein aggregation below the freezing temperature. 10th International Conference on Protein Stabilization.
2014. Apresentação oral de trabalho. Accelerated Formulation Studies for Frozen Storage of Proteins. 9th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology.
2013. Apresentação oral de trabalho. Live-Cell imaging FRET reveals clustering of the prion protein at the cell surface induced by infectious prions. 2nd Iberian Congress on Prions.
2013. Apresentação oral de trabalho. Stabilization of FMN-dependent NADPH:dye/quinone reductase from Pseudomonas putida MET94 by directed evolution. 9th European Biophysical Congress.
2013 - 2013. Organização de evento. Head of the organizing committee (2013). Universidade do Algarve.
2013 - 2013. Participação em evento. Chairman of the “Protein Folding” session. Congresso. 9th European Biophysical Congress.
2013 - 2013. Orientação. Catálise de pontes dissulfureto pelas vias enzimáticas que actuam no retículo endoplasmático usando como substrato a proteína fluorescente HyPer.Biological Engineering (Mestrado). Orientador.
2013 - 2013. Orientação. Neurotoxicidade de fibrilhas da proteína priónica.Biological Engineering (Mestrado). Orientador.
2012. Apresentação oral de trabalho. Detection and Neurotoxicity of Prions. 1st Iberian Congress on Prions.
2012. Apresentação oral de trabalho. Protein fibrillation, osmolytes and prion diseases. 9th International Conference on Protein Stabilization.
2012 - 2012. Arbitragem científica em conferência. 1st Iberian Congress on Prions. Santiago de Compostela, Espanha.
2012 - 2012. Participação em evento. Chairman at the 9th International Conference on Protein Stabilisation. 9th International Conference on Protein Stabilisation.
2012 - 2012. Orientação. Fibrillation and neurotoxicity of the prion protein.Biological Engineering (Mestrado). Orientador.
2012 - 2012. Orientação. Oxidative protein folding in the endoplasmic reticulum.Biological Engineering (Mestrado). Orientador.
2011 - 2011. Curso / Disciplina lecionado. Global approach to proteomics. Universidade do Algarve.
2011. Apresentação oral de trabalho. Protein Fibrillation, Prion Diseases and the Origin of Life. I Ciclo de Conferências – Núcleo de Estudantes de Ciências Biomédicas.
2010 - 2010. Curso / Disciplina lecionado. Folding kinetics and Stability. Protein folding, misfolding and disease, 9th short course of the Portuguese Biophysical Society (Especialização pós-licenciatura).
2010. Apresentação oral de trabalho. Stability of CotA – laccase from Bacillus subtilis and Metallo-Oxidase McoA from Aquifex aeolicus to thermal and chemical stability. Oxidative Enzymes as Sustainable Industrial Biocatalysts.
2010. Apresentação oral de trabalho. Insight into the stability of multicopper oxidases. Protein-Protein Interactions and Protein folding. Universidade Nova de Lisboa (Lisboa, Portugal).
2010. Apresentação oral de trabalho. Insight into the stability of multicopper oxidases. Journées Jeunes Chercheurs.
2010. Apresentação oral de trabalho. Insight into the stability of multicopper oxidases. Oxizymes & 9th International Symposium of Peroxidases.
2010. Apresentação oral de trabalho. Insight into the stability of multicopper oxidases. 3rd Annual World Congress of Industrial Biotechnology – IBIO 2010.
2005 - 2010. Orientação. Prion-Diseases: A Biophysical approach to the pathological protein conformer.Biological Sciences (Doutoramento). Orientador.
2009. Apresentação oral de trabalho. Protein Fibrillation is prevented by Osmolytes. 8th International Conference on Protein Stabilization.
2005 - 2009. Orientação. Structure-Function relationships and stability of extremophilic lacases.Biochemistry (Doutoramento). Coorientador.
2009 - 2009. Orientação. Prião Murinico: Produção, separação, mutagenese e estabilidade à autólise.Biological Engineering (Mestrado). Orientador.
2008. Apresentação oral de trabalho. Folding and amyloidosis of proteins: The prevention of amyloidosis by osmolytes. XVIth National Congress of Biochemistry.
2008. Apresentação oral de trabalho. Fluorescence studies of the pair CFP-YFP: FLIM-FRET in vitro studies and expression in neuronal cells to probe the prion protein at the cell surface. Focus on Microscopy.
2008. Apresentação oral de trabalho. Copper incorporation into recombinant CotA-laccase from Bacillus subtilis. Oxizymes, 4th European meeting in Oxizymes.
2007 - 2007. Curso / Disciplina lecionado. Proteins at the Cell Surface Probed by Fluorescence Lifetime Imaging Microscopy. Portugal-MIT program on Bioengineering (Especialização pós-licenciatura). Universidade de Lisboa Instituto Superior Técnico.
2007. Apresentação oral de trabalho. Insight Into the Stability of Multi-Copper Oxidases. 7th International Conference on Protein Stabilization.
2003 - 2007. Orientação. Protein Stabilization by media engineering and directed evolution.Biotechnology (Doutoramento). Orientador.
2006. Apresentação oral de trabalho. Priões. Forum da Química. Universidade Nova de Lisboa (Lisboa, Portugal).
2006. Apresentação oral de trabalho. Enzyme Stabilization: different strategies for different enzymes. 6th European Symposiun on Biochemical Engineering Science.
2006. Apresentação oral de trabalho. Perturbations of the T1 copper site in the CotA-laccase from Bacillus subtilis: Structural, Biochemical, Enzymatic and Stability Studies. Eurobic8. 8th European Biological Inorganic Chemistry Conference.
2006. Apresentação oral de trabalho. Enzymatic properties, structural insights and stability of a highly efficient metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus. Copper 06. 5th International Copper Meeting.
2005. Apresentação oral de trabalho. Protein Folding and Stabilization by Osmolytes . International Conference on Enzyme Technology, RELATENZ 2005.
2005 - 2005. Participação em evento. Chairman at the International Conference on Enzyme Technology, RELATENZ 2005. Congresso. International Conference on Enzyme Technology, RELATENZ 2005.
2001 - 2005. Orientação. Folding and Stability of Proteins in the presence of Low Molecular Weight Solutes.Biotechnology (Doutoramento). Orientador de Chen Luyang.
2004. Apresentação oral de trabalho. Effect of Trehalose on Folding and Stability of the Ribosomal Protein S6 . 6th International Conference on Protein Stabilization.
2004 - 2004. Participação em evento. Chairman of the 6th International Conference on Protein Stabilization. Congresso. 6th International Conference on Protein Stabilization.
2000 - 2004. Orientação. Conformational Stability Studies of Two Anionic Horseradish Peroxidases Isoenzymes.Biotechnology (Doutoramento). Coorientador.
2001. Apresentação oral de trabalho. Cutinase Folding and Stabilization by Trehalose. 10th European Congress on Biotechnology.
2001 - 2001. Orientação. Improving thermal/structural stability of cutinase in the presence of low molecular weight solutes and by directed evolution.Biotechnology (Mestrado). Orientador.
2000 - 2000. Participação em evento. Chairman of the Protein Folding session. Congresso. 2nd International Conference on Protein Stabilization.
1999 - 1999. Curso / Disciplina lecionado. Lipase interactions with Micelles. Structure, Function and Stability of Lipases (Doktor (PhD)). Aalborg Universitet.
1997. Apresentação oral de trabalho. Deactivation Equilibria for Cutinase in Reverse Micelles. 8th European Congress on Biotechnology.
Projetos
Projetos
2017 - 2018. Desenvolvimento de Tecnologia Criogénica para Substâncias Biológicas, Portugal 2020. Investigador. Universidade do Algarve.
2013/04 - 2015/03. Functionalized Metal Nano-Gaps for Plasmon-Enhanced Single Protein Biosensing. Investigador.
2012/02 - 2015/01. Bioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis. Investigador.
2012/02 - 2015/01. A Biophysical Approach to Oxidative Protein Folding in the ER: Relative Importance of Ero1 and Prdx4 alternative pathways. Investigador responsável.
2010/01 - 2013/03. Controlled freezing to improve the stability and delivery of therapeutic proteins. Investigador.
2008/04 - 2012/03. Single Molecule Signalling of Peptide Metal Binding. Investigador.
2008/04 - 2011/12. Single-Molecule Detection of the Prion Protein: Characterization of Conformational Change Involved on Prion Diseases. Investigador responsável.
2008/03 - 2011/12. Photoinduced electron transfer by artificial hemoproteins in carbon nanotubes. Investigador.
2004/01/15 - 2008. Design of Stabilizing Affinity Molecular Probes : A Rational Approach to Assess and Enhance Protein Stability, POCI.
2005/06 - 2008/11. A Murine Neuroblastoma Cell-Based Approach to Study and Prevent Prion Diseases. Investigador responsável.
2005/10 - 2008/06. Lipid-Lipid and Protein-Lipid Interactions in Model Systems of Cell Membranes. Investigador.
2005/09 - 2008/06. Molecular organization of functionalized porphyrins in giant vesicles and supramolecules. Investigador.
2003/03/15 - 2006. Relationship between CYP3A4 gene polymorphisms, protein structure and activity in disease setting, POCI.
Produções
Melo, Eduardo P. 2014. "PROCESSO E APARELHO DE CONGELAÇÃO EM PRATOS PARA OBTENÇÃO DE MATRIZES DE GELO COM DISTRIBUIÇÃO DE SOLUTOS UNIFORME". Portugal. Concedida/Emitida.
Melo, Eduardo Pinho; Konno, Tasuku; Farace I.; Awadelkareem, M. A.; Skov, L.R.; Teodoro, F.; Sancho, T.P.; et al. Autor correspondente: Avezov, Edward. 2022. "Stress-induced protein disaggregation in the Endoplasmic Reticulum catalysed by BiP". Nature Commun.
Tasuku Konno; Eduardo Pinho Melo; Joseph E. Chambers; Edward Avezov. 2021. "Intracellular Sources of ROS/H2O2 in Health and Neurodegeneration: Spotlight on Endoplasmic Reticulum". Cells, 10 (2): 233-233. https://doi.org/10.3390/cells10020233
Correia, Cátia; Tavares, Evandro; Lopes, Carlos; Silva, Joana G.; Duarte, Andreia; Geraldes, Vitor; Rodrigues, Miguel A.; Melo, Eduardo P.. 2020. "Stability of Protein Formulations at Subzero Temperatures by Isochoric Cooling". Journal of Pharmaceutical Sciences, 109 (1): 316-322. https://doi.org/10.1016/j.xphs.2019.06.017
David Botequim; Inês I. R. Silva; Sofia G. Serra; Eduardo P. Melo; Duarte M. F. Prazeres; Sílvia M. B. Costa; Pedro M. R. Paulo. 2020. "Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement". Nanoscale, 12: 6334-6345. https://doi.org/10.1039/D0NR00267D
Martins, Lígia O.; Melo, Eduardo Pinho; Sanchez-Amat, Antonio; Robalo, Maria Paula. 2020. "Bacterial Laccases: Some Recent Advances and Applications". Em Laccases in Bioremediation and Waste Valorisation, 27-55. Springer International Publishing. https://doi.org/10.1007/978-3-030-47906-0_2
Pfluck ACD; de Barros DPC; Fonseca LP; Melo EP. 2018. "Stability of lipases in miniemulsion systems: Correlation between secondary structure and activity.". Enzyme and microbial technology, 114: 7-14. https://doi.org/10.1016/j.enzmictec.2018.03.003
Macedo JA; Schrama D; Duarte I; Tavares E; Renaut J; Futschik ME; Rodrigues PM; Melo EP. 2017. "Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells.". https://doi.org/10.1186/s12864-017-3694-6
Melo EP; Lopes C; Gollwitzer P; Lortz S; Lenzen S; Mehmeti I; Kaminski CF; Ron D; Avezov E. 2017. "TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2.". https://doi.org/10.1186/s12915-017-0367-5
Anjos L; Morgado I; Guerreiro M; Cardoso JC; Melo EP; Power DM. 2017. "Cartilage acidic protein 1, a new member of the beta-propeller protein family with amyloid propensity.". https://doi.org/10.1002/prot.25210
Eduardo Pinho Melo; Carlos Lopes; Peter Gollwitzer; Stephan Lortz; Sigurd Lenzen; Ilir Mehmeti; Clemens F. Kaminski; David Ron; Edward Avezov. 2016. TriPer, an optical probe tuned to the endoplasmic reticulum tracks H2O2 consumption by glutathione. https://doi.org/10.1101/088351
Konno, Tasuku; Pinho Melo, Eduardo; Lopes, Carlos; Mehmeti, Ilir; Lenzen, Sigurd; Ron, David; Avezov, Edward. 2015. "ERO1-independent production of H2O2 within the endoplasmic reticulum fuels Prdx4-mediated oxidative protein folding". The Journal of Cell Biology, 211 (2): 253-259. https://doi.org/10.1083/jcb.201506123
Estrela N; Franquelim HG; Lopes C; Tavares E; Macedo JA; Christiansen G; Otzen DE; Melo EP. 2015. "Sucrose prevents protein fibrillation through compaction of the tertiary structure but hardly affects the secondary structure.". http://europepmc.org/abstract/med/26344410
Avezov E; Konno T; Zyryanova A; Chen W; Laine R; Crespillo-Casado A; Melo EP; et al. 2015. "Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum.". https://doi.org/10.1186/s12915-014-0112-2
Tavares, Evandro; Macedo, Joana A.; Paulo, Pedro M.R.; Tavares, Catarina; Lopes, Carlos; Melo, Eduardo P.. 2014. "Live-cell FRET imaging reveals clustering of the prion protein at the cell surface induced by infectious prions". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1842 (7): 981-991. https://doi.org/10.1016/j.bbadis.2014.02.002
Brissos, Vânia; Gonçalves, Nádia; Melo, Eduardo P.; Martins, Lígia O.. 2014. "Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution". PLoS ONE, 9 (1). https://doi.org/10.1371/journal.pone.0087209
Tsunoda, Satoshi; Avezov, Edward; Zyryanova, Alisa; Konno, Tasuku; Mendes-Silva, Leonardo; Pinho Melo, Eduardo; Harding, Heather P; Ron, David. 2014. "Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants". eLife, 3. https://doi.org/10.7554/elife.03421
Anjos, Liliana; Gomes, Ana S.; Melo, Eduardo P.; Canário, Adelino V.; Power, Deborah M.. 2013. "Cartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding protein". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1834 (3): 642-650. https://doi.org/10.1016/j.bbapap.2012.12.012
Avezov, E.; Cross, B.C.S.; Schierle, G.S.K.; Winters, M.; Harding, H.P.; Melo, E.P.; Kaminski, C.F.; Ron, D.. 2013. "Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox". Journal of Cell Biology, 201 (2): 337-349. https://doi.org/10.1083/jcb.201211155
Rosa, M.; Lopes, C.; Melo, E.P.; Singh, S.K.; Geraldes, V.; Rodrigues, M.A.. 2013. "Measuring and modeling hemoglobin aggregation below the freezing temperature". Journal of Physical Chemistry B, 117 (30): 8939-8946. https://doi.org/10.1021/jp4035369
Fernandes, A.T.; Lopes, C.; Martins, L.O.; Melo, E.P.. 2012. "Unfolding pathway of CotA-laccase and the role of copper on the prevention of refolding through aggregation of the unfolded state". Biochemical and Biophysical Research Communications, 422 (3): 442-446. https://doi.org/10.1016/j.bbrc.2012.05.011
Fernandes, A.T.; Pereira, M.M.; Silva, C.S.; Lindley, P.F.; Bento, I.; Melo, E.P.; Martins, L.O.. 2011. "The removal of a disulfide bridge in CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability". Journal of Biological Inorganic Chemistry, 16 (4): 641-651. https://doi.org/10.1007/s00775-011-0768-9
Zito, E.; Melo, E.P.; Yang, Y.; Wahlander, Å.; Neubert, T.A.; Ron, D.. 2010. "Oxidative Protein Folding by an Endoplasmic Reticulum-Localized Peroxiredoxin". Molecular Cell, 40 (5): 787-797. https://doi.org/10.1016/j.molcel.2010.11.010
Melo, E.P.; Estrela, N.; Lopes, C.; Matias, A.C.; Tavares, E.; Ochoa-Mendes, V.. 2010. "Compacting proteins: Pros and cons of osmolyte-induced folding". Current Protein and Peptide Science, 11 (8): 744-751. https://doi.org/10.2174/138920310794557727
Fernandes, A.T.; Martins, L.O.; Melo, E.P.. 2009. "The hyperthermophilic nature of the metallo-oxidase from Aquifex aeolicus". Biochimica et Biophysica Acta - Proteins and Proteomics, 1794 (1): 75-83. https://doi.org/10.1016/j.bbapap.2008.09.006
Madeira, C.; Estrela, N.; Ferreira, J.A.B.; Andrade, S.M.; Costa, S.M.B.; Melo, E.P.. 2009. "Fluorescence lifetime imaging microscopy and fluorescence resonance energy transfer from cyan to yellow fluorescent protein validates a novel method to cluster proteins on solid surfaces". Journal of Biomedical Optics, 14 (4). https://doi.org/10.1117/1.3210770
Durão, P.; Chen, Z.; Fernandes, A.T.; Hildebrandt, P.; Murgida, D.H.; Todorovic, S.; Pereira, M.M.; Melo, E.P.; Martins, L.O.. 2008. "Copper incorporation into recombinant CotA laccase from Bacillus subtilis: Characterization of fully copper loaded enzymes". Journal of Biological Inorganic Chemistry, 13 (2): 183-193. https://doi.org/10.1007/s00775-007-0312-0
Baptista, R.P.; Pedersen, S.; Cabrita, G.J.M.; Otzen, D.E.; Cabral, J.M.S.; Melo, E.P.. 2008. "Thermodynamics and mechanism of cutinase stabilization by trehalose". Biopolymers, 89 (6): 538-547. https://doi.org/10.1002/bip.20926
Brissos, V.; Melo, E.P.; Martinho, J.M.G.; Cabral, J.M.S.. 2008. "Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT". Biochimica et Biophysica Acta - Proteins and Proteomics, 1784 (9): 1326-1334. https://doi.org/10.1016/j.bbapap.2008.04.017
Morgado, I.; Melo, E.P.; Lundberg, E.; Estrela, N.L.; Sauer-Eriksson, A.E.; Power, D.M.. 2008. "Hormone affinity and fibril formation of piscine transthyretin: The role of the N-terminal". Molecular and Cellular Endocrinology, 295 (1-2): 48-58. https://doi.org/10.1016/j.mce.2008.06.010
Chen, Z.; Franco, C.F.; Baptista, R.P.; Cabral, J.M.S.; Coelho, A.V.; Rodrigues Jr., C.J.; Melo, E.P.. 2007. "Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides". Applied Microbiology and Biotechnology, 73 (6): 1306-1313. https://doi.org/10.1007/s00253-006-0605-1
Otzen, D.E.; Giehm, L.; Baptista, R.P.; Kristensen, S.R.; Melo, E.P.; Pedersen, S.. 2007. "Aggregation as the basis for complex behaviour of cutinase in different denaturants". Biochimica et Biophysica Acta - Proteins and Proteomics, 1774 (2): 323-333. https://doi.org/10.1016/j.bbapap.2006.11.012
Carvalho, A.S.L.; Ferreira, B.S.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Melo, E.P.. 2007. "Thermal denaturation of HRPA2: pH-dependent conformational changes". Enzyme and Microbial Technology, 40 (4): 696-703. https://doi.org/10.1016/j.enzmictec.2006.05.029
Viseu, M.I.; Melo, E.P.; Carvalho, T.I.; Correia, R.F.; Costa, S.M.B.. 2007. "Unfolding kinetics of ß-lactoglobulin induced by surfactant and denaturant: A stopped-flow/fluorescence study". Biophysical Journal, 93 (10): 3601-3612. https://doi.org/10.1529/biophysj.106.101667
Melo, E.P.; Fernandes, A.T.; Durão, P.; Martins, L.O.. 2007. "Insight into stability of CotA laccase from the spore coat of Bacillus subtilis". Biochemical Society Transactions, 35 (6): 1579-1582. https://doi.org/10.1042/BST0351579
Neves-Petersen, Maria Teresa; Klitgaard, Søren; Carvalho, Ana Sofia Leitão; Petersen, Steffen B.; Aires de Barros, Maria Raquel; e Melo, Eduardo Pinho. 2007. "Photophysics and Photochemistry of Horseradish Peroxidase A2 upon Ultraviolet Illumination". Biophysical Journal, 92 (6): 2016-2027. https://doi.org/10.1529/biophysj.106.095455
Chen, L.; Ferreira, J.A.B.; Costa, S.M.B.; Cabrita, G.J.M.; Otzen, D.E.; Melo, E.P.. 2006. "Compaction of ribosomal protein S6 by sucrose occurs only under native conditions". Biochemistry, 45 (7): 2189-2199. https://doi.org/10.1021/bi051490g
Durão, P.; Bento, I.; Fernandes, A.T.; Melo, E.P.; Lindley, P.F.; Martins, L.O.. 2006. "Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: Structural, biochemical, enzymatic and stability studies". Journal of Biological Inorganic Chemistry, 11 (4): 514-526. https://doi.org/10.1007/s00775-006-0102-0
Pedersen, S.; Nesgaard, L.; Baptista, R.P.; Melo, E.P.; Kristensen, S.R.; Otzen, D.E.. 2006. "pH-dependent aggregation of cutinase is efficiently suppressed by 1,8-ANS". Biopolymers, 83 (6): 619-629. https://doi.org/10.1002/bip.20598
Rodrigues, M.L.; Archer, M.; Martel, P.; Miranda, S.; Thomaz, M.; Enguita, F.J.; Baptista, R.P.; et al. 2006. "Crystal structures of the free and sterol-bound forms of ß-cinnamomin". Biochimica et Biophysica Acta - Proteins and Proteomics, 1764 (1): 110-121. https://doi.org/10.1016/j.bbapap.2005.09.008
Carvalho, A.S.L.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Pinho E Melo, E.. 2005. "Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium". Biochimica et Biophysica Acta - Proteins and Proteomics, 1747 (1): 99-107. https://doi.org/10.1016/j.bbapap.2004.10.002
Chen, L.; Cabrita, G.J.M.; Otzen, D.E.; Melo, E.P.. 2005. "Stabilization of the ribosomal protein S6 by trehalose is counterbalanced by the formation of a putative off-pathway species". Journal of Molecular Biology, 351 (2): 402-416. https://doi.org/10.1016/j.jmb.2005.05.056
Carvalho, A.S.L.; Santos, A.M.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Pinho E Melo, E.. 2004. "Conformational states of HRPA1 induced by thermal unfolding: Effect of low molecular weight solutes". Biopolymers, 75 (2): 173-186. https://doi.org/10.1002/bip.20100
Melo, E.P.; Chen, L.Y.; Cabral, J.M.S.; Fojan, P.; Petersen, S.B.; Otzen, D.E.. 2003. "Trehalose favors a cutinase compact intermediate off-folding pathway". Biochemistry, 42 (24): 7611-7617. https://doi.org/10.1021/bi034267x
Baptista, R.P.; Chen, L.Y.; Paixão, A.; Cabral, J.M.S.; Melo, E.P.. 2003. "A novel pathway to enzyme deactivation: The cutinase model". Biotechnology and Bioengineering, 82 (7): 851-857. https://doi.org/10.1002/bit.10641
Melo, E.P.; Baptista, R.P.; Cabral, J.M.S.. 2003. "Improving cutinase stability in aqueous solution and in reverse micelles by media engineering". Journal of Molecular Catalysis B: Enzymatic, 22 (5-6): 299-306. https://doi.org/10.1016/S1381-1177(03)00044-4
Carvalho, A.S.L.; Melo, E.P.E.; Ferreira, B.S.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.. 2003. "Heme and pH-dependent stability of an anionic horseradish peroxidase". Archives of Biochemistry and Biophysics, 415 (2): 257-267. https://doi.org/10.1016/S0003-9861(03)00275-3
Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.; Fojan, P.; Petersen, S.B.. 2003. "Cutinase - AOT interactions in reverse micelles: The effect of 1-hexanol". Chemistry and Physics of Lipids, 124 (1): 37-47. https://doi.org/10.1016/S0009-3084(03)00031-8
Melo, Eduardo P. 2003. "Estabilidade de Proteínas". Em Engenharia Enzimática. LIDEL.
Melo, E.P.; Faria, T.Q.; Martins, L.O.; Gonalves, A.M.; Cabral, J.M.S.. 2001. "Cutinase unfolding and stabilization by trehalose and mannosylglycerate". Proteins: Structure, Function and Genetics, 42 (4): 542-552. https://doi.org/10.1002/1097-0134(20010301)42:4<542::AID-PROT120>3.0.CO;2-4
Melo, E.P.; Aires-Barros, M.R.; Cabral, J.M.S.. 2001. "Reverse micelles and protein biotechnology". Biotechnology Annual Review, 7: 87-129. https://doi.org/10.1016/S1387-2656(01)07034-X
Melo, E.P.; Fojan, P.; Cabral, J.M.S.; Petersen, S.B.. 2000. "Dynamic light scattering of cutinase in AOT reverse micelles". Chemistry and Physics of Lipids, 106 (2): 181-189. https://doi.org/10.1016/S0009-3084(00)00152-3
Melo, E.P.; Taipa, M.A.; Castellar, M.R.; Costa, S.M.B.; Cabral, J.M.S.. 2000. "A spectroscopic analysis of thermal stability of the Chromobacterium viscosum lipase". Biophysical Chemistry, 87 (2-3): 111-120. https://doi.org/10.1016/S0301-4622(00)00171-X
Baptista, R.P.; Cabral, J.M.S.; Melo, E.P.. 2000. "Trehalose delays the reversible but not the irreversible thermal denaturation of cutinase". Biotechnology and Bioengineering, 70 (6): 699-703. https://doi.org/10.1002/1097-0290(20001220)70:6<699::AID-BIT13>3.0.CO;2-N
Carvalho, C.M.L.; Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.; Aires-Barros, M.R.. 1999. "A steady-state fluorescence study of cutinase microencapsulated in AOT reversed micelles at optimal stability conditions". Biotechnology Letters, 21 (8): 673-681. https://doi.org/10.1023/A:1005550213632
Melo, E.P.; Carvalho, C.M.L.; Aires-Barros, M.R.; Costa, S.M.B.; Cabral, J.M.S.. 1998. "Deactivation and conformational changes of cutinase in reverse micelles". Biotechnology and Bioengineering, 58 (4): 380-386. https://doi.org/10.1002/(SICI)1097-0290(19980520)58:4<380::AID-BIT5>3.0.CO;2-F
Pinho Melo, E.; Aires-Barros, M.R.; Costa, S.M.B.; Cabral, J.M.S.. 1997. "Thermal unfolding of proteins at high pH range studied by UV absorbance". Journal of Biochemical and Biophysical Methods, 34 (1): 45-59. https://doi.org/10.1016/s0165-022x(97)01202-5
Carneiro-da-Cunha, M.G.; Melo, E.P.; Cabral, J.M.S.; Aires-Barros, M.R.. 1996. "Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles". Bioprocess Engineering, 15 (3): 151-157. https://doi.org/10.1007/s004490050249
Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.. 1996. "Denaturation of a recombinant cutinase from Fusarium solani in AOT-iso-octane reverse micelles: A steady-state fluorescence study". Photochemistry and Photobiology, 63 (2): 169-175. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030038712&partnerID=MN8TOARS
Ransac, Stéphane; Carrière, Frédéric; Rogalska, Ewa; Verger, Robert; Marguet, Frank; Buono, Gérard; Melo, Eduardo Pinho; et al. 1996. "The Kinetics, Specificities and Structural Features of Lipases". Em Engineering of/with Lipases, 143-182. Springer Netherlands. https://doi.org/10.1007/978-94-009-1671-5_10
Carneiro-Da-Cunha, M. G.; Melo, E. P.; Sebastião, M. J.; Aires-Barros, M. R.; Cabral, J. M. S.. 1996. "Separation and Purification of Lipases by Liquid-Liquid Extraction Processes". Em Engineering of/with Lipases, 551-576. Springer Netherlands. https://doi.org/10.1007/978-94-009-1671-5_35
Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.. 1995. "Structural effect of reversed micelles of AOT over a recombinant cutinase from Fusarium solani pisi - A steady state fluorescence study". Annals of the New York Academy of Sciences, 750: 85-88. https://doi.org/10.1111/j.1749-6632.1995.tb19931.x
Setti, L.; Fevereiro, P.; Melo, E.P.; Pifferi, P.G.; Cabral, J.M.S.; Aires-Barros, M.R.. 1995. "Superactivity of peroxidase solubilized in reversed micellar systems". Applied Biochemistry and Biotechnology, 55 (3): 207-218. https://doi.org/10.1007/BF02786860
Melo, E. Pinho; Ivanova, Margarita G.; Aires-Barros, M. Raquel; Cabral, Joaquim M. S.; Verger, Robert. 1995. "Glyceride synthesis catalyzed by cutinase using the monomolecular film technique". Biochemistry, 34 (5): 1615-1621. https://doi.org/10.1021/bi00005a018
Melo, E. P.; Airesbarros, M. R.; Cabral, J. M. S.. 1995. "Triglyceride hydrolysis and stability of a recombinant cutinase fromFusarium solani in AOT-iso-octane reversed micelles". Applied Biochemistry and Biotechnology, 50 (1): 45-56. https://doi.org/10.1007/bf02788039
Barreto, M. T. O.; Melo, E. P.; Almeida, J. S.; Xavier, A. M. R. B.; Carrondo, M. J. T.. 1991. "A kinetic method for calculating the viability of lactic starter cultures". Applied Microbiology and Biotechnology, 34 (5): 648-652. https://doi.org/10.1007/bf00167916
Barreto, M. T. O.; Melo, E. P.; Moreira, J. L.; Carrondo, M. J. T.. 1991. "High cell density reactor for the production of Lactobacillus plantarum". Journal of Industrial Microbiology, 7 (1): 63-69. https://doi.org/10.1007/bf01575605
Barreto, M. T. O.; Melo, E. P.; Carrondo, M. J. T.. 1989. "Starter culture production in fluidized bed reactor with a flocculent strain of L. plantarum". Biotechnology Letters, 11 (5): 337-342. https://doi.org/10.1007/bf01024514
